標準培養限制有幾種生理和病理過程，其中細胞經歷體積變化。然而，沒有可靠的方法可用于實時準確測量貼壁細胞的體積。 細胞體積測定的優勢細胞在光學透明的腔室中培養，該腔室能夠間準確確定它們的體積，并并行跟蹤導致體積變化的生化過程，例如離子泵的激活。 例子從間期到 Raji 細胞有絲分裂的體積跟蹤 [2]。
（A） 將細胞置于由柱設定的校準高度的聚（二甲基硅氧烷）室中，在補充有FITC葡聚糖的培養基中。下圖：細胞在熒光圖像上排除熒光（比例尺20 mm）。 （B） 對應于（A）中虛線的熒光文件：熒光強度的大值和小值分別對應于腔室大高度（背景）和零高度（柱）。右圖：這些值用于校準信號并計算細胞的光學厚度。 （C） 后，通過對細胞面積上的總熒光強度進行積分來獲得細胞體積。 (A) HeLa 細胞的體積軌跡。開始和結束時的兩個體積超調對應于有絲分裂中的瞬時體積增加，一個對應于母細胞，二個對應于子細胞。 (B) (A) 中使用 FXm 的細胞的原始熒光圖像。比例尺 50 毫米。
參考[1] Zlotek-Zlotkiewicz, E. 等人。(2015)。細胞生物學雜志，211 (4)，765–774。 [2] Cadart, C 等人。(2017)。細胞生物學方法，139,103-120。
提供實現工具細胞限制器-cell confiner（Cell confinement） 我們的cell Confiner是一種多功能設備，可通過對細胞應用定義明確的約束條件來研究細胞力學。限制方法基于將細胞固定在兩個平行表面之間，從而實現均勻且定義明確的物理參數，例如細胞幾何形狀和環境彈性。此外，可以使用高分辨率顯微鏡對受限的細胞進行成像，因為該設備是光學透明的，并將細胞保持在焦平面上。 細胞被均勻地限制/壓縮在兩個亞微米分辨率的兩個平行表面之間。兩個表面之間的空間由微型PDMS支柱控制。 微型支柱在載玻片上制造，載玻片連接到PDMS活塞（吸盤）上。 活塞由真空泵控制，因此限制區的高度也受到控制。不同的限制高度（例如1um – 300um），允許長期細胞培養和細胞增殖，同時保持對封閉的完美控制
與高分辨率光學顯微鏡系統兼容，可以處理足夠多的細胞以進行完整的基因表達分析，可與生物功能化的微結構化底物和/或不同的基質（幾何形狀控制）結合使用
可以與凝膠結合（硬度控制），兼容任何細胞培養底物（培養皿至96孔板）。 產品特性： >定義細胞的厚度和形狀 用正確的限制滑片控制細胞的厚度 >同時進行多個實驗 能夠研究不同的細胞或同時應用不同的限制條件 >適用于高分辨率顯微鏡 光學透明的材料和緊湊的設計可實現高分辨率顯微鏡 >控制限制速度 通過真空泵j確控制限制速度 >可逆限制：限制后取回您的細胞 由于細胞的非破壞性方法，可以進行分子分析 >與您自己的實驗兼容 該限制器是一種小型設備，直接放置在您的細胞培養液頂部 技術方案圖
三種產品形式典型應用：>癌癥浸潤測定：遷移行為和遷移轉變的量化 >癌癥侵襲性測定：體細胞或癌細胞的收縮力定量 >內吞作用測定：更好地觀察膜發生的事件 >胞吐法測定：更好地觀察在頂端膜發生的事件 >吞噬功能失調：機制的表征 >孔中的免疫系統：非粘附免疫細胞的二維遷移和相互作用 >免疫細胞相互作用：非貼壁免疫細胞的2D相互作用 >有絲分裂組裝測定：有絲分裂紡錘體疾病的定量 >定量細胞遷移測定：細胞遷移特性的快速，精細分析 >癌癥研究 轉移細胞的遷移 轉移中的細胞收縮 DNA DSB修復（機械誘導） 基因組不穩定（細胞分裂） 分離共培養 >免疫學 免疫細胞遷移 非粘附細胞的成像 >器官生理學 癌細胞遷移 具有硬度控制的細胞區分 傷口愈合 分離共培養 細胞壓縮反應 >罕見疾病 細胞核完整性 >老化 細胞核完整性 自噬相關疾病 >觀測化 非粘附細胞的成像 細胞器的平面成像 >基礎研究 細胞體積（細胞周期） 細胞機械力刺激反應 二維心肌細胞成熟測定
二維肝小管化驗
3D心肌細胞成熟測定
3D肝小管測定
附著球體測定
細胞收縮力測定
細胞遷移測定
細胞核擠壓測定
細胞j化
細胞體積測量
趨化性測定
共培養測定
胞吞試驗
胞吐法
外泌體測定
片狀脂蛋白和絲狀體含量測定
活細胞成像
巨噬細胞j化測定
MT依賴性運輸測定
神經肌肉連接測定
井中的神經元網絡
細胞器定位分析
初次纖毛測定
骨骼肌細胞測定
平滑肌細胞
傷口愈合測定
PUBLICATIONS - Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672 - Actin flows induce a universal coupling between cell speed and cell persistence
P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386 - Geometric friction directs cell migration
M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101 - Mitotic rounding alters cell geometry to ensure efficient spindle assembly
O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283 - Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414 - Exploring the Function of Cell Shape and Size during Mitosis
C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169 - Methods for Two-Dimensional Cell Confinement
M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29
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相關產品： 1、細胞力產生和力傳遞綜合測量分析系統 2、各種生物力學設備
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