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Isoprostanes are primarily generated by free radical mechanisms making them excellent biomarkers for systemic oxidant stress. It is now known that isoporostanes are extensively metabolized in humans. Oxford Biomedical Research has found that an average of 50% of the isoprostane excreted in human urine is conjugated to glucuronic acid. The extent of glucuronidation among individuals ranges significantly from 28% to 80%. In light of this information it is strongly recommended that specimens be pretreated with b-glucuronidase prior to isoprostane analysis to provide a more accurate assessment of oxidative stress.

This kit provides methods and sufficient materials for the treatment of 40 samples allowing the user to differentiate the inter-individual differences in glucuronidation and measure the total systemic isoprostane output.
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